PCR-Dye question?

hoolwerf at solair1.inter.nl.net hoolwerf at solair1.inter.NL.net
Wed Jun 25 09:30:15 EST 1997


Hello,

mbi071 at sysc.abdn.ac.uk (j.r.stephen) wrote:

 jrs> I have seen an advertisment at some point for a dye you add to
 jrs> PCR mixes to see a +/­ reaction, ie. it only fluoresces if you
 jrs> get a product. Does anyone know what I'm talking about, or am I
 jrs> mixing­dreams and reality again?

It's reality. <g>

We used from Perkin Elmer the TaqMan Salmonella kit and it's a PCR system in
which fluorescens is produced together with the product of the primer set used.

You can get info from PE of course. The drawback of this method is that, as far
as i know, you can only obtain the double labeled probe you need from PE and
it's not really cheap. :­)


with regards, Jan Hoolwerf

******************* these words were mine, all mine !! ********************
 J.D. Hoolwerf
 Neth. Inst. for Dairy Research
 P.O. Box 20                        phone  : 31­318­659511
 6710 BA  Ede                       fax    : 31­318­650400
 the Netherlands                    e­mail : hoolwerf at solair1.inter.nl.net
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