100bp ladder

Lars Andrup andrup at internet.dk
Wed Jun 25 12:47:22 EST 1997


Dear Huang Zhong
I got the following answer from Life Technologies:

The 100 Bp DNA Ladder is derived from the plasmid, pSS246.  pSS246 (5672
bp) was
constructed by cloning two Stu I cassettes (each containing 15; 100 bp
repeats)
into the MCS of pUC.  The ladder is generated by completely digesting
pSS246 w/
Ssp I and Stu I.  A partial digest w/ EcoR V is done subsequent to the
complete
digests, to liberate the 100 bp repeats.

Source of bands:
  2072 bp.......................pUC
  600 bp........................actually it is a mix of a 600 bp
fragment
                                of pUC from the Ssp I, Stu I digest and
a
                                600 bp frag from EcoR V partial.
 100 bp repeats.................each 100 bp frag is comprised up of 20
bases
                                of a synthetic sequence and 80 bases
from the
                                1,000 bp repeat (yeast 2 u gene
sequence,
                                coordinates 291 to 378).


Huang Zhong wrote:

> Dear netters,
>
> Who know how to get the sequence information about commercial 100bp
> ladder? Any clues appreciated.
>
> Huang zhong
> --
> --------------------------------------K.U.Leuven---------------------
> HUANG Zhong
> Lab of Gut Hormone        => http://urc1.cc.kuleuven.ac.be/~m9631766/
> Gasthuisberg, B-3000      => e-mail: huang.zhong at med.kuleuven.ac.be
> Leuven, Belgium           => Fax: 32-16-345939     Tel: 32-16-345750
> ---------------------------------------------------------------------




Yours

Lars
--
*********** ooo O ooo ***********
Lars Andrup, senior researcher
National Institute of Occupational Health
Lersø Parkallé 105, DK-2100 Copenhagen, Denmark
phone:+45 3916-5223 fax: +45 3916-5201
*********** ooo O ooo ***********





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