Sodium or Ammonium?

Richard L lm11 at gpu.srv.ualberta.ca
Tue Jun 24 15:15:49 EST 1997


Dear colleagues,

I want to recover soem DNA fragments from gels for cloning, but I don't
know which salt, sodium or ammonium acetate, should be used to
precipaitate DNA (along with ethanol). Some people say ammonium aceate is
more effective for precipitation, but the trace of ammonium will inhibit
the ligation reaction, is that true?

Thanks a lot

Richard





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