pcr with NH4+

Ned Mantei mantei at neuro.biol.nospam.ethz.ch
Thu Jun 26 07:44:09 EST 1997


In article <moellere.15.00A2F12D at pz350.ipsp.uni-hohenheim.de>,
moellere at pz350.ipsp.uni-hohenheim.de (EVELYN MOELLER) wrote:

> Hello:
> 
> I would like to know if anyone on the net has experience with including NH4 
> ions (additional to MgCl2) into the PCR buffer and knows which concentrations 
> are useful....?

Our 1x buffer contains:
16.6 mM ammonium sulfate
67.7 mM TRIS-HCl, pH 8.89
10 mM beta-mercaptoethanol
170 micrograms/ml BSA
as much magnesium chloride as is appropriate for the particular
amplification (generally 1.5--3 mM)

The name associated with this sort of buffer is Gitschier, but I can't
find a literature reference for it. Also we find that this can work better
than the buffer the suppliers offer.

-- 
Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch   Fax: +41-1-633-1046

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