Looking for dam-/dcm- bact. strain
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Thu Jun 26 03:28:47 EST 1997
In article <rocco-2306971359260001 at haarerlab.zo.utexas.edu>, Steve
Palmieri <rocco at mail.utexas.edu> writes
>I'm in dire need of a dcm-/dam- bacterial strain for cloning purposes. I
>have a construct with an ApaI site that is undergoing methylation(there is
>a DCM methylation site via sequence analysis). I've tried EB2007 but I
>think our glycerol stock may be bad because they won't transform. If
>anyone has a methylation defective bacterial strain that they had success
>with it will be much appreciated. Thanks.
E.coli B - all B strains are dcm-
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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