pcr with NH4+

Jerry Kropp jkropp at itsa.ucsf.edu
Thu Jun 26 14:50:34 EST 1997


In article <drm21-ya023080002606971648190001 at nntp-serv.cam.ac.uk>,
drm21 at mole.bio.cam.ac.uk (David Micklem) wrote:

(snip)
> 
> If you have a look at recent posts (eg with www.dejanews.com), you may find
> mention of betaine as a new wonder-additive, and the references I think.
> Does anyone know if this is whats in Qiagen's new buffer.....
> 
> David
> 
David,
(We MUST stop meeting like this. People will talk...)
I used betaine @60mM in an ampl. of a gnarley 70% G/C template and
increased the robustness of the reaction at least 4-fold, based on the
amt. of product appearing on my gel.
Haven't tried it on a "normal" template, however.
Cheers,
Jerry

"I have concommitant amnesia and deja vu. I think I forgot this before."



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