RNA from unfertilized eggs

M. Aitchison m.j.aitchison at ncl.ac.uk
Fri Jun 27 13:25:17 EST 1997


hi netters,

when i purify total RNA from unfertilized sea urchin eggs, there's some other 
stuff that copurifies and shows up on the EtBr stained formaldehyde/agarose 
gel. However, it runs the other way, towards the cathode. can anyone outhere
tell me how to get rid of this (it seems to interfere with mRNA isolation)? 
LiCl precipitation alone does not help!

Thanks an awful lot, M.




More information about the Methods mailing list