glycerol - mode of actions ?

Keld Sorensen KeldS at uic.edu
Fri Jun 27 05:54:16 EST 1997


Part of the action of glycerol is that it prevents freezing - PBS and
similar buffers are notorious for dropping a couple of pH values when
they freeze. To see this for yourself, simply add a drop of "Universal
indicator" to a PBS buffer, then freeze it and look at the color.

Glycerol and ethylene glycol are both used. 

Note that some qualitites of glycerol and ethylene glycol have reactive
impurities.

As to other "protein stabilizing" effects, they are both creating a
polyhydroxy environment that some enzymes and proteins "like"

Keld.



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