rising antibodies

Martin Offterdinger a8803349 at unet.univie.ac.at
Mon Jun 30 08:30:30 EST 1997

On Mon, 30 Jun 1997 11:55:52 -0700, Alberto Piccin <AP12 at le.ac.uk>

>Dear netters,
>	I am planning to rise a rabbit polyclonal antibody against a 
>protein fragment of about 300 aa which is expressed as a GST fusion.
>Browsing through several protocols did not give much help in deciding a 
>suitable strategy. Therefore I would greatly appreciate your opinions 
>and expertise.
>In the manual by Harlow and Lane, they reckon that antigens in 
>particulate form are more antigenic than soluble proteins, therefore I 
>was thinking to use for the first boost the glutathione-agarose beads 
>bound to my fusion protein and then only use the 300 aa fragment for the 
>successive boosts.
>Any comments or suggestions? Any idea of antigen dosage or whether the  
>agarose beads will also work as an adjuvant?
>Thanks in advance
Please avoid posting your message 14(!!!) times. We are all going to
understand it even if it is posted only once!

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