a8803349 at unet.univie.ac.at
Mon Jun 30 08:30:30 EST 1997
On Mon, 30 Jun 1997 11:55:52 -0700, Alberto Piccin <AP12 at le.ac.uk>
> I am planning to rise a rabbit polyclonal antibody against a
>protein fragment of about 300 aa which is expressed as a GST fusion.
>Browsing through several protocols did not give much help in deciding a
>suitable strategy. Therefore I would greatly appreciate your opinions
>In the manual by Harlow and Lane, they reckon that antigens in
>particulate form are more antigenic than soluble proteins, therefore I
>was thinking to use for the first boost the glutathione-agarose beads
>bound to my fusion protein and then only use the 300 aa fragment for the
>Any comments or suggestions? Any idea of antigen dosage or whether the
>agarose beads will also work as an adjuvant?
>Thanks in advance
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