Single Copy Plasmids

daniel jacobs d.jacobs at unsw.edu.au
Sun Mar 2 23:49:52 EST 1997


In article <bbeitzel-2602971242340001 at willsmac2.ucsd.edu>,
bbeitzel at jeeves.ucsd.edu (Brett Beitzel) wrote:

> Anyone know of any commercially available single copy E. coli plasmids? 
> If not, what oris will maintain a plasmid as a single copy?
> 
> Just Wondering,
> Brett Beitzel
> bbeitzel at jeeves.ucsd.edu

Try the BAC vector pBeloBAC11. It is single copy (f factor origin) and you
can obtain it from a number of sources. The best place to try is probably
http://www.tree.caltech.edu/. It is a bit of a pain to purify so if you
need a lot send me an email as I maybe able to help. Good luck.



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