differentiating between genomic & plasmid DNA

Scott Kuhns kuhns.scott at mayo.edu
Mon Mar 3 19:22:54 EST 1997


What's wrong with equilibrium dinesity centrifugation?

martinez at puccini.CRL.umn.edu (J. Pat Martinez) wrote:

>One way to eliminate non-supercoiled DNA is to use Dnase Dependent DNAse
>from epicentre.  This enzyme will only degrade linear DNA, leaving the
>supercoiled DNA intact.

>Pat M.


>> E.Hillery (e.hillery at ic.ac.uk) wrote:
>> : Would anybody out there have any thoughts on how I could differentiate
>> : between eukaryotic genomic and pg amounts of plasmid DNA.  In an ideal
>> : world, I wouls like to separate the two species so that I could then
>> : quantify the latter
>> 
>> : many thanks
>> : Liz Hillery
>> : Ion Transport                                  'phone: 171 352 8121 x3393
>> : National Heart & Lung Institute                 fax: 171 351 8340
>> : Manresa Road                                    e-mail:E.Hillery at ic.ac.uk
>> : London
>> : SW3 6LR





More information about the Methods mailing list