Tli polymerase for PCR...

Sai siyer at bmg.bhs.uab.edu
Tue Mar 4 15:36:01 EST 1997


hey all,
          i'm trying to use Tli polymerase (from promega) to do PCR
instead of taq to avoid the addition of the extra nucleotide at the end of
my PCR products.  has anybody used this polymerase before for PCR?  the
data sheet says that it has the same activity conditions as taq and uses
the same buffers etc.  the enzyme is also exceptionally thermostable. 
anybody know of any special steps/protocols to take when using this for
PCR?  please email to sai at uab.edu.  thanks...

-- 
Sai Iyer (sai at uab.edu)
Pre-doctoral fellow i.e lowly graduate student (Dr.Gerald Hart's lab)
Dept of Biochemistry and Molecular Genetics
University of Alabama at Birmingham...



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