in situ hybridization

William wwu at shriners.mcgill.ca
Tue Mar 4 12:58:39 EST 1997


Hi, I'm doing in situ hybridization of cartilage. The problem is that
the cyro sections do not stay on the slide. I've used all kinds of
slides such poly lysine, superfrost, probe on, you name it. A collegue
told me to incubate the sections with the slides for 2 hrs at 60C. I've
done that and indeed the sections stay on, but I'm afraid there might be
degradation of the rna. Does anybody else have a better way.
Thanks

William



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