Single Copy Plasmids

someone somewhere at universe.com
Tue Mar 4 13:05:41 EST 1997



Brett Beitzel <bbeitzel at jeeves.ucsd.edu> wrote in article
<bbeitzel-2602971242340001 at willsmac2.ucsd.edu>...
> Anyone know of any commercially available single copy E. coli plasmids? 
> If not, what oris will maintain a plasmid as a single copy?
 

I am not aware of any commercially available single-copy vectors, but I
believe that F and RP4 (RK2) plasmids are maintained at 1-2 copies per
cell.  Some (rather large) vectors were constructed using replicons of
these plasmids in the past.  Do a Medline search.

M.A.



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