pre-made Northern blots

Julie Roberts jroberts at rascal.med.harvard.edu
Tue Mar 4 11:12:01 EST 1997


Francois Coulier wrote:
> 
> On 4 Mar 1997, Kohta Takahashi wrote:
> 
> > I am looking to purchase a commercial Northern blot containing human
> > tissue samples. I am aware that Clontech and Invitrogen sell them. Are
> > there other companies which also sell these? Which is better? I would
> > appreciate any help or advice that anyone can offer.
> >
> > Kohta Takahashi
> >
> > Kohta.Takahashi at molbio.unige.ch
> >
> We have been using Clontech's Multiple Tissue Northern, and are quite
> satisfied with them, although RNA loading may vary from one membrane to
> the next. This means you need to hybridize control probe(s)  to each
> membrane. We have hybridized them 5-6 times, so far, with no significant
> loss in signal intensity.
> 
> *  *  *  *  *  *  *  *  *  *  *  *  *
> Francois Coulier

We have also used MTNs from Clonetech tho we have been using the mouse
ones, we have been very happy with our results!

J. Roberts
jroberts at rascal.med.harvard.edu



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