A little help here...

[Hany Saqr]

In article <helsayed-0403971534040001 at path4.med.ohio-state.edu>, We use
MEM to grow several glioma cell lines.  After we add EAA, NEAA and
vitamoins.  we buffer the medium using 1 M NaOH to get pH 7.2-7.4 (around
5 ml). You could also add NaHco3 instead of NaOH.,cheers wrote:

> In article <5f86nc$jmo at isnews.csc.calpoly.edu>,
> smccull at jurassic.fisher.calpoly.edu (The Kindly One) wrote:
> 
> > Maybe someone can help me here. I have made Minimum Essential Medium
> > for a fibroblast cell line and the papers from NIGMS call for
> > 2x essent and non-essen amino acids. If I make the media at
> > pH 7.2 so that it's ~7.4 after filtering, then addition of
> > the AA drop sthe medi to a bright yellow color that I'm sure is
> > too acidic to be healthy. Any ideas(besides adding AA before filtering)
> > 
We use MEM to grow several glioma cell lines.  After we add EAA, NEAA and
vitamoins.  we buffer the medium using 1 M NaOH to get pH 7.2-7.4 (around
5 ml). You could also add NaHco3 instead of NaOH.,cheers
We use MEM to grow several glioma cell lines.  After we add EAA, NEAA and vitamoins.  we buffer the medium using 1 M NaOH to get pH 7.2-7.4 (around 5 ml). You could also add NaHco3 instead of NaOH.,cheers 

-- 
Hany Saqr M.D., Ph.D.
Pathology Depat
OSU
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