A little help here...
helsayed at magnus.acs.ohio-state.edu
Tue Mar 4 15:30:36 EST 1997
In article <5f86nc$jmo at isnews.csc.calpoly.edu>,
smccull at jurassic.fisher.calpoly.edu (The Kindly One) wrote:
> Maybe someone can help me here. I have made Minimum Essential Medium
> for a fibroblast cell line and the papers from NIGMS call for
> 2x essent and non-essen amino acids. If I make the media at
> pH 7.2 so that it's ~7.4 after filtering, then addition of
> the AA drop sthe medi to a bright yellow color that I'm sure is
> too acidic to be healthy. Any ideas(besides adding AA before filtering)
> Thanks sdm
> ** Scott D. McCulloch(Bunny Lover) | If we knew what we were doing,
> ** smccull at jurassic.fisher.calpoly.edu | it would not be called
> ** BioChemical Effects Research | research, now would it?
> ** http://www.calpoly.edu/~smccullo/ |
Hany Saqr M.D., Ph.D.
helsayed at pop.service.ohio-state.edu
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