A little help here...

Hany Saqr helsayed at magnus.acs.ohio-state.edu
Tue Mar 4 15:30:36 EST 1997


In article <5f86nc$jmo at isnews.csc.calpoly.edu>,
smccull at jurassic.fisher.calpoly.edu (The Kindly One) wrote:

> Maybe someone can help me here. I have made Minimum Essential Medium
> for a fibroblast cell line and the papers from NIGMS call for
> 2x essent and non-essen amino acids. If I make the media at
> pH 7.2 so that it's ~7.4 after filtering, then addition of
> the AA drop sthe medi to a bright yellow color that I'm sure is
> too acidic to be healthy. Any ideas(besides adding AA before filtering)
> 
> Thanks sdm
> 
> -- 
> ** Scott D. McCulloch(Bunny Lover)      | If we knew what we were doing,
> ** smccull at jurassic.fisher.calpoly.edu  | it would not be called     
> ** BioChemical Effects Research         | research, now would it?
> ** http://www.calpoly.edu/~smccullo/    |

-- 
Hany Saqr M.D., Ph.D.
Pathology Dep.
OSU
helsayed at pop.service.ohio-state.edu



More information about the Methods mailing list