Paraformaldehyde and autofluorescence.
immunolo at COURRIER.USHERB.CA
Wed Mar 5 17:09:16 EST 1997
I perform immunophenotyping of human lymphocytes by flow cytometry.
I use 1% paraformaldehyde solution (PFA; Sigma) in PBS for cell fixation.
The autofluorescence of fixed cell exited at 488nm is increased 6-7 fold
comparing to nonfixed control.
1. Can someone suggest me an alternative PFA source which gives
2. Can someone suggest me a post-fixation protocol which reduces
PFA-fixed cell autofluorescence?
Dr. L. Volkov
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