Uracil containing DNA and Proof-reading pols

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed Mar 5 04:09:34 EST 1997

Hi Folks,

There have been a few posts recently about the use of Pfu or Vent etc
with uracil containing primers or using dUTP in with the nucleotides. As
I just came across a paper in my filing cabinet about this it may be of

Lasken et al J.Biol.Chem Vol 271 No. 30, pp17692-17696, (1996)

The work was done by Life Technologies and basically shows that  uaracil
containing DNA binds with 6500 fold greater infinity than non-uracil
containing DNA so effectively acts as an inhibitor. dUTP as a nucleotide
was incorporated with approx 40% reduced rate. 

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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