how do restriction endonuclweases work?
wgschech at med.uni-tuebingen.de
Wed Mar 5 06:27:44 EST 1997
Hi all there! No, don' tell me: "they cut DNA at specific sites".
What I want to know is: HOW DO THEY FIND THIS SITE!
Are they walking along the DNA (where do they bind initially?) until
they think that it might be a good place to bite or are they
attracted magically by the restriction site thus they approach
like from outer space and hit the DNA just at the right place?
What happens after the cut? Does the RE fall off immediately or
does it still remain attached to the DNA (how much space -base
pairs?- do they consume) and will it -in the end- be able to block
another -different species- of RE to cut near this site?
Just my last nightmare.
I'm keen your comments and ideas!
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University of Tuebingen
email: wgschech at med.uni-tuebingen.de
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