Tli polymerase for PCR...

Dr. Duncan Clark duncan at
Wed Mar 5 04:02:10 EST 1997

In article <siyer-0403971536010001 at>, Sai
<siyer at> writes
>hey all,
>          i'm trying to use Tli polymerase (from promega) to do PCR
>instead of taq to avoid the addition of the extra nucleotide at the end of
>my PCR products.  has anybody used this polymerase before for PCR?  the
>data sheet says that it has the same activity conditions as taq and uses
>the same buffers etc.  the enzyme is also exceptionally thermostable. 
>anybody know of any special steps/protocols to take when using this for
>PCR?  please email to sai at  thanks...

It's the same enzyme as VENT from NEB.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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