Tli polymerase for PCR...

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed Mar 5 04:02:10 EST 1997


In article <siyer-0403971536010001 at bmg146.cmc.uab.edu>, Sai
<siyer at bmg.bhs.uab.edu> writes
>hey all,
>          i'm trying to use Tli polymerase (from promega) to do PCR
>instead of taq to avoid the addition of the extra nucleotide at the end of
>my PCR products.  has anybody used this polymerase before for PCR?  the
>data sheet says that it has the same activity conditions as taq and uses
>the same buffers etc.  the enzyme is also exceptionally thermostable. 
>anybody know of any special steps/protocols to take when using this for
>PCR?  please email to sai at uab.edu.  thanks...
>


It's the same enzyme as VENT from NEB.

Duncan
--------------------------------------------------------------------------------
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Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
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