EtBr staining of DNA

David Micklem drm21 at mole.bio.cam.ac.uk
Thu Mar 6 13:38:42 EST 1997


In article <331F08DD.6A8 at niehs.nih.gov>, NIEHS, Mail, Drop, F2-02,
Research, Triangle, Park, NC, 27709 wrote:


>I add EtBr to both my gel soln and my sample when i am doing quick and
>dirty gels.  This really helps when trying to see small (100-200 bp) DNA
>which otherwise gets kind of fuzzy on an agarose gel.  I use a final
>conc of 10 ug/ml in the gel, and I add EtBr to my loading buffer so that
>its conc is also 10 ug/ml in the loading buffer.  works like a charm. 
>Add the EtBr spiked loading buffer to your DNA std, too, to prevent
>worries about aberrant runs.
>
>Caroline Seay

Wow, thats a _lot_.  I add more like 0.25ug/ml (1/10000 of 2.5mg/ml stock)
in the gel (and the gel buffer).  

David

-- 
D.R.Micklem,                                Time flies like an arrow... 
Wellcome/CRC Institute,            Fruit flies like a banana.       
Cambridge CB2 1QR, UK               Tel: [+44] (0)1223 334129
Email:drm21 at mole.bio.cam.ac.uk Fax: [+44] (0)1223 334089  



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