EtBr staining of DNA

[Marieke R. Koedood Zhao]

If it's for 'quick and dirty', why don't you just add EtBr to your agarose
gel? We add EtBr to 0.5 or 1ug/ml in our agarose solution just before pouring
the gel. DNA stains as runs thru gel Only occasionally need to re-stain -
when gel ran too long and EtBr front (running opposite to DNA) has passed 
the smaller bands. Most of the time no problem.

Marieke


Mike (m3allen at sciborg.uwaterloo.ca) wrote:
: Hello netters:
: Does anyone have an opinion and/or protocol for staining DNA in an
: agarose gel by adding EtBr directly to the sample?
: I know it can change how the DNA runs.  This would be more for quick &
: dirty gels.
: Thanks in advance.
: Mike
: m3allen at sciborg.uwaterloo.ca