Adaptor construction

charles lawrie charles.lawrie at trinity.ox.ac.uk
Thu Mar 6 06:37:07 EST 1997


Please help me . I have two adaptors to make from complementary 
oligonucleotides ( a 43mer + 11mer and a 44mer + 8mer )by annealing 
together . My question is at what temp do I anneal and under what 
conditions and is there any way to verify the annealing . Please reply 
by e-mail if you have any ideas or possible references .

Thanks alot and Cheers!:}



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