sodium acetate in sequencing gel buffers!

[cynthia stryker]

zarir (zarir at dept.agry.purdue.edu) wrote:
: Hi people,
: i want to know the reason for adding sodium acetate to the lower buffer
: tank while doing manual sequencing. I know it reduces compressions, by
: altering the voltage gradient, but how? what is the chemistry involved?
: Please reply urgently.also some reference where it is documented.
: thanks,
: zarir E.V.
: dept. of agronomy,
: purdue university.
: zarir at dept.agry.purdue.edu

Biotechniques, vol 6, # 10 (1988)  Cindy at UNL