Cloning with Tth 111 I

Jose Gonzales imnr at
Wed Mar 5 12:16:39 EST 1997

Hello all!

I am forced to clone with Tth 111 I. I know that cloning with this
enzyme is extremely inefficient. Does anyone out there have any tips
for cloning with this or any 1 base overhang enzyme?

Thanks in advance!

Jose E. N. Gonzales
The Immune Response Corporation

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