Sequencing and sharkteeth

Mike Gruidl mgruidl at com1.med.usf.edu
Thu Mar 6 20:50:15 EST 1997


Edwin Abeln wrote:
> 
> In article <330C80D5.1A01 at nfh.uit.no>, Jørgen Stenvik <joergens at nfh.uit.no> says:
> >
> >We have some trouble with leakage using sharkteeth combs for sequencing.
> >What can be done?
> 
> Afer you poored your gel and inserted your sharkteeth comb, you can
> put bulldog paper clamps over the comb and both glasplates during
> the polymerisation.
> 
> cheers Edwin


I use the clamps but I also apply a VERY thin coat of silicone grease or 
petroleum jelly (like vasoline) to the teeth before inserting the comb. 
 Clean up with methanol or ethanol.

Regards

Mike gruidl



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