Sequencing and sharkteeth

Mike Gruidl mgruidl at
Thu Mar 6 20:50:15 EST 1997

Edwin Abeln wrote:
> In article <330C80D5.1A01 at>, Jørgen Stenvik <joergens at> says:
> >
> >We have some trouble with leakage using sharkteeth combs for sequencing.
> >What can be done?
> Afer you poored your gel and inserted your sharkteeth comb, you can
> put bulldog paper clamps over the comb and both glasplates during
> the polymerisation.
> cheers Edwin

I use the clamps but I also apply a VERY thin coat of silicone grease or 
petroleum jelly (like vasoline) to the teeth before inserting the comb. 
 Clean up with methanol or ethanol.


Mike gruidl

More information about the Methods mailing list