Hirt procedure pl.

[Rick Nicholson]

> We wish to employ Hirt procedure to isolate plasmid DNA from the genomic 
> DNA of cultured cells. We have read the original paper of Hirt 1967. I am 
> sure lot more of you will have great tips and may be modifications that 
> would be of great help. We shall greatly appriciate your comments and 
> suggestions. I am sure your experinces will help us avoid pit-falls. 
> Please try and be as explanatory as possible. Thanks in advance.
> Jawahar.
> 

I would be interested to see tips on this as well.  Could you forward
any tips you get on to me please.
Thanks, Rick.
-- 
Richard C. Nicholson, BSc PhD
Senior Research Fellow
Centre for Immunology
St. Vincent's Hospital
Sydney, Australia 2010