FISH multicolour background

Geoff Scopes adbio at adbio.demon.co.uk
Mon Mar 10 04:35:15 EST 1997


In article <3320581C.6762 at sheffield.ac.uk>, Gahtani <M.H.Algahtani at sheff
ield.ac.uk> writes
>Hi...
>
>I am dong multicolour FISH Exp. on lymphoma chrmosome metaphases ( 
>patients and cell lines) using Teaxes Red and FITC ( direct and indirect 
>tech,) but the problem which I am facing know is the background 
>specially with FITC, larg and tiny green dots all over the slides
>
>please, I need any help to cont. my work, S who has the magic answer.
>
>looking to read your answer on my e-mail.
>
>thanks
>
>Qahtani
Are you doing the FITC labelling indirect? If so you could try
centrifuging your Fluorescein-Avidin (we do this after we have added it
to blocking buffer). This is because the Fluorescein Avidin forms
aggregates during storage.


Regards,
--                                                       ______
Dr Geoff Scopes,
Research and Development Manager,               
Units B1-B2, Longmead Business Centre,
Blenheim Road,
Epsom,
Surrey KT19 9QQ, UK.
Tel:  01372 723 456             E.mail: geoff at adbio.demon.co.uk
Fax:  01372 741 414             Web Site:  http://www.adbio.co.uk/




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