pETor pGEX expression => opinions requested.

Chris Kafer ckafer at iastate.edu
Mon Mar 10 18:10:12 EST 1997


I have an approximately 55 kD Arabidopsis protein I want to express in
E. coli.  My choices seem to be the pGEX GST fusion system or the pET
His tag system.  I want to use this protein for crystallization. 

If you have experience with these two sytems I would love to hear your
opinions on the suitability of each.  Also of interest would be ease
of purification and purity of final protein obtained.  

Thanks!


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Chris Kafer	www.public.iastate.edu/~ippm	       PGP Key available
		www.public.iastate.edu/~thorn
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