Afinity purification with MAb - kits, protocols?

D. Pearton pearton at saul7.u.washington.edu
Mon Mar 10 19:18:11 EST 1997


Hi all,

I have a mouse monoclonal antibody -IgG1- (in ascities fluid) wich I would
like to use to affinity purify the antigen from cell lysates using a
column method (protein A beads?). Does anyone have suggestions for
commercially available kits or a reference to a protocol that I could look
at.  I know the antibody can be readily purified with the MapsII kit but I
need some system to use so I can run my tissue extract over it.

Responses via email preferred.

Thanks in advance,
--
Dave Pearton
pearton at u.washington.edu
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Down he sank in a chair-ran his hands through his hair-	+
     And chanted in mimsiest tones			+
Words whose utter inanity proved his insanity,		+
     While he rattled a couple of bones.		+
							+
"The Hunting of the Snark" Lewis Carroll		+
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++



More information about the Methods mailing list