Afinity purification with MAb - kits, protocols?
pearton at saul7.u.washington.edu
Mon Mar 10 19:18:11 EST 1997
I have a mouse monoclonal antibody -IgG1- (in ascities fluid) wich I would
like to use to affinity purify the antigen from cell lysates using a
column method (protein A beads?). Does anyone have suggestions for
commercially available kits or a reference to a protocol that I could look
at. I know the antibody can be readily purified with the MapsII kit but I
need some system to use so I can run my tissue extract over it.
Responses via email preferred.
Thanks in advance,
pearton at u.washington.edu
Down he sank in a chair-ran his hands through his hair- +
And chanted in mimsiest tones +
Words whose utter inanity proved his insanity, +
While he rattled a couple of bones. +
"The Hunting of the Snark" Lewis Carroll +
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