desperately need help

Bob Steinberg RSTEINBE at ETOWAH.UOKHSC.EDU
Mon Mar 10 20:50:24 EST 1997


AAAACc wrote:
> 
> Hi people who are expert for western!
> 
> I am doing western using PY20 (antiphosphotyrosine antibody) and
> developing with ECL  but it did not work. I checked every condition it is
> still not working.
> 
>  Any suggestion is appreciated.
> 
> Thank you

My lab has only limited experience with ECL, but you should beware that 
with ECL either too much signal or too little signal can give you a blank 
film.  Apparently, if the signal is too strong, all the substrate can be 
exhausted before you get your film onto the membrane.



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