desperately need help
RSTEINBE at ETOWAH.UOKHSC.EDU
Mon Mar 10 20:50:24 EST 1997
> Hi people who are expert for western!
> I am doing western using PY20 (antiphosphotyrosine antibody) and
> developing with ECL but it did not work. I checked every condition it is
> still not working.
> Any suggestion is appreciated.
> Thank you
My lab has only limited experience with ECL, but you should beware that
with ECL either too much signal or too little signal can give you a blank
film. Apparently, if the signal is too strong, all the substrate can be
exhausted before you get your film onto the membrane.
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