western blot

Keld Sorensen KeldS at uic.edu
Mon Mar 10 08:21:08 EST 1997

Background in Western Blot is often due to too high level
of the enzyme-antibody conjugate. Just dilute it and see
what happens - usually the specific signal is fine and the
background disappears. But it all depends - you need to
give more specific information, i.e. have you done ANY
troubleshooting at all??

More information about the Methods mailing list