UV crosslinking

Alexander Kraev kraev at bc.biol.ethz.ch
Tue Mar 11 11:05:10 EST 1997

In article <Pine.GSO.3.96.970311140600.29030A-100000 at sun1.oulu.fi>, Pete
<pkursula at cc.oulu.fi> wrote:

> OK, OK... So I've done it years ago but now I need a reminder:
> When you crosslink DNA with UV light onto 1) nitrocellulose 2) nylon
> membrane, does the membrane have to be: 1) wet 2) almost wet 3) dry.
Either way works, but the optimal time is about double for the wet one
relative to the dry one.


Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
Phone 41-1-632-31-47
Fax 41-1-632-12-13
e-mail kraev at bc.biol.ethz.ch

More information about the Methods mailing list