UV crosslinking

Alexander Kraev kraev at bc.biol.ethz.ch
Tue Mar 11 11:05:10 EST 1997


In article <Pine.GSO.3.96.970311140600.29030A-100000 at sun1.oulu.fi>, Pete
<pkursula at cc.oulu.fi> wrote:

> OK, OK... So I've done it years ago but now I need a reminder:
> 
> When you crosslink DNA with UV light onto 1) nitrocellulose 2) nylon
> membrane, does the membrane have to be: 1) wet 2) almost wet 3) dry.
> 
Either way works, but the optimal time is about double for the wet one
relative to the dry one.

Cheers,

-- 
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
Phone 41-1-632-31-47
Fax 41-1-632-12-13
e-mail kraev at bc.biol.ethz.ch



More information about the Methods mailing list