G-banding mouse spreads

[Susan Hogarth]

W R Bennett wrote:
> 
> Does anyone know what sort of variables might control whether G-bands are
> visible on mouse chromosome spreads?  I ran 4 slides simultaneously
> through G-banding yesterday, and one slide shows beautiful banding, and
> the rest don't - the chromosomes are evenly stained with Giemsa
> throughout.  The spreads were made from different flasks of E15 embryonic
> fibroblasts, but were all made on the same day ...

So much for the "phase-of-the-moon effect" ;-)