Which strand does T7 RNA pol transcribe from?

[Peter Stoilov]

You are right that T7 RNA polymerase uses the antisense strand for
template. However T7 RNA polymerase could transcribe the second strand
of your DNA if it's 3'-end is singlestranded. You can find some details
here:

Nucleic Acids Res 13: 6223-6236 (1985) 
A novel transcription property of SP6 and T7 RNA
polymerases: dependence on template structure.
Schenborn ET, Mierendorf RC Jr 
-- 
Peter Stoilov
Bulgarian Academy of Sciences
Institute of Molecular Biology
G. Bonchev str, Bld. 21
1113 Sofia
Bulgaria

Phone: (+359 2) 713 2651
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E-mail: pstoilov at obzor.bio21.acad.bg