UV crosslinking

miketrower at aol.com miketrower at aol.com
Tue Mar 11 17:32:41 EST 1997

In article <Pine.GSO.3.96.970311140600.29030A-100000 at sun1.oulu.fi>, Pete <pkursula at cc.oulu.fi> writes:

>OK, OK... So I've done it years ago but now I need a reminder:
>When you crosslink DNA with UV light onto 1) nitrocellulose 2) nylon
>membrane, does the membrane have to be: 1) wet 2) almost wet 3) dry.
>I think our manual for the Stratalinker (no affiliation) is missing.

We have worked with hundreds of Nylon membranes and have always crosslinked (on a Stratalinker) dry - no problems!


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