UV crosslinking

[Levon Fendekian]

Pete <pkursula at cc.oulu.fi> wrote:

>OK, OK... So I've done it years ago but now I need a reminder:
>
>When you crosslink DNA with UV light onto 1) nitrocellulose 2) nylon
>membrane, does the membrane have to be: 1) wet 2) almost wet 3) dry.
>
>I think our manual for the Stratalinker (no affiliation) is missing.
>
>Pete
>

I think it should be a little humid ... Not dry !