Blunt-end troubles

Brenda Gerhardt bgerhar at farmr1.med.uth.tmc.edu
Tue Mar 11 06:39:30 EST 1997


I had a similar experience with a sticky end ligation where I wanted
both orientations of a BamHI insert. In this situation, I could never
get the opposite orientation. I was using blue/white selection and found
that when I didn't use IPTG/Xgal I was able to get the second
orientation. I am not sure if this is something that you are doing, but
maybe it will help.

Good luck,
Brenda



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