PCR product in wells

Wayne M. Barnes wayne at BARNES1.WUSTL.EDU
Tue Mar 11 12:51:12 EST 1997


Dear Astrid,

      I asked Bruno Zimm what kind of DNA would stick in the wells, since
total E.coli and intact yeast chromosomes do not stick in wells, and he
said it must be something with more than 2 ends.

      If the product has 3 or more ends, these ends will hang up on the
agarose and be unable to serpentate (I think that's the word).  

      That's all I have (or don't have, as the case may be).  

Wayne M. Barnes, Ph.D.                        wayne at barnes1.wustl.edu
Biochemistry Dept. 8231                   or  barnes at biodec.wustl.edu
Washington Univ. Medical School               314.362.3351  fax 7183
660 South Euclid Ave., St. Louis, MO 63110
           http://mbb.wustl.edu/~barnes/

> Dear Dr. Barnes,
> 
> Thank you very much for your mail from last week. It was very 
> interesting ! 
> But I still don t understand exactly what kind of contaminations 
> could create PCR products that are so big that they stay in the well 
> during electrophoresis.
> Do you have an explaination for this phenomenon?
> Please write me again.
> 
> Best regards, 
> Astrid Slany
> 




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