ribonuclease protection assay and non-isotopic label

e. lader lader at uts.cc.utexas.edu
Tue Mar 11 12:33:17 EST 1997


In article <727ABC44580 at wvuhsc1.hsc.wvu.edu> Lennie Samsell,
lsamsell at WVUHSC1.HSC.WVU.EDU writes:
>   Please pardon me if this is an old question, but I am new at this. 
>Does anyone have experience using non-isotopic labeling with a 
>ribonuclease protection assay? I am aware that Ambion claims that 
>their non-isotopic kit can be used with their RPA II kit with no loss 
>in sensitivity. If anyone has had that experience or one contrary to 
>it  I would really appreciate hearing about it. 

We have been doing quite a number of experiments addressing this issue.
The answer is yes, in our hands, the non isotopic system is as sensitive. 

Being an old 32P guy, I would rather skip the electoblot,  the price of
the membrane, and the biotin and the HRP, but to each his own.

lader at ambion.com



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