Apparati for Northern Blotting

e. lader lader at uts.cc.utexas.edu
Tue Mar 11 12:26:29 EST 1997


In article <dhynds-2602971835500001 at path9.med.ohio-state.edu> DiAnna L.
Hynds, dhynds at pop.service.acs.ohio-state.edu writes:
>>I am in the market for a system for Northern blotting.  I am planning on
>getting a 12 x 14 cm horizontal electrophoresis system and would like
>opinions on this.  Also I am not sure what would be the best blotting
>system to use.  I have seen electrophoretic transfer, passive (upward or
>downward) transfer and vacuum blotting.  I would really appreciate any
>opinions on which system people like and any preferred manufacturers. 
>Thank you in advance.

Either electroblot or  transfer without compressing the gel. Also
alkaline transfer onto positively charged membranes.

Call Ambion at 800-888-8804 and request some literature on Northern
Blotting.

Eric Lader
lader at ambion.com



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