Adaptor construction

e. lader lader at uts.cc.utexas.edu
Tue Mar 11 12:23:30 EST 1997


In article <5fmaj8$sgq at news.ox.ac.uk> charles lawrie,
charles.lawrie at trinity.ox.ac.uk writes:
>Please help me . I have two adaptors to make from complementary 
>oligonucleotides ( a 43mer + 11mer and a 44mer + 8mer )by annealing 
>together . My question is at what temp do I anneal and under what 
>conditions and is there any way to verify the annealing . Please reply 
>by e-mail if you have any ideas or possible references .
>
>Thanks alot and Cheers!:}

Try 100 mM NaCl/TE. Try it at a concentration of like 100 ng/ul. Put in
beaker of 90 degree water, let cool to room temp.

Always works. 

lader at ambion.com



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