UV crosslinking

Rafael Maldonado rafael at howard.genetics.utah.edu
Wed Mar 12 16:17:02 EST 1997


On Tue, 11 Mar 1997, Levon Fendekian wrote:

> Pete <pkursula at cc.oulu.fi> wrote:
> 
> >OK, OK... So I've done it years ago but now I need a reminder:
> >
> >When you crosslink DNA with UV light onto 1) nitrocellulose 2) nylon
> >membrane, does the membrane have to be: 1) wet 2) almost wet 3) dry.

Nylon and dry. Nitrocellulose does not work and water can shield UV 
radiation.



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