UV crosslinking

[Rafael Maldonado]

On Tue, 11 Mar 1997, Levon Fendekian wrote:

> Pete <pkursula at cc.oulu.fi> wrote:
> 
> >OK, OK... So I've done it years ago but now I need a reminder:
> >
> >When you crosslink DNA with UV light onto 1) nitrocellulose 2) nylon
> >membrane, does the membrane have to be: 1) wet 2) almost wet 3) dry.

Nylon and dry. Nitrocellulose does not work and water can shield UV 
radiation.



___________________________________________________________________
                                             |
Rafael Maldonado                             | 'No te creas todo 
room 6160 Eccles Institute of Human Genetics |  lo que leas en
Department of Human Genetics		     |  internet.'
University of Utah			     |
Salt Lake City, Utah 84112. USA.	     |  "Don't believe  
Rafael.Maldonado at genetics.utah.edu           |  everything you read
Rafael at howard.genetics.utah.edu	             |  in internet."
Tel: 801-581-4429			     |
Fax: 801-585-3910			     |