mmm_klehnert at mednov1.auckland.ac.nz
Wed Mar 12 21:02:39 EST 1997
does anyone out there have a good (and tried) protocol for
Tris-Tricine Polyacrylamide gels?
I have tried the protocols in the "original" publication
(Schaeger+Jagow 1987), I'm using high-quality reagents, but at
the very best I'm getting very fuzzy bands, and even when I run
the gel (in a BioRad Mini-ProteanII) for twice as long (i.e.
duoble the time the bluemarker needs to run out), my smallest
marker band (Aprotinin, 6.5kDa) is still in the upper half of
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