Are PCR reactions stable at 4 degrees?
Harry.Witchel at Bristol.Ac.Uk
Thu Mar 13 08:18:26 EST 1997
Bonjour mes amis Moleculaire !
I was wondering if anyone knew how stable PCR reactions were when
stored in the refrigerator at 4 degrees. I know that if I do a PCR run
overnight and use that reaction as a template for a further reaction, I
will get the correct product, but I seem to be getting results suggesting
that if I take the first reaction and store it in the refrigerator for
3-4 days (as a PCR reaction, with oil and all the reactants still in it,
I can't do the second half of the reaction (the result is usually a
smear with no indication of the correct band). Does anyone else have
experience of this problem? Is there some way to protect the reaction
simply (eg adding EDTA) or do I have to remove the oil and phenol
chloroform extract every single reaction I want to reuse?
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