l.kempster at ic.ac.uk
Thu Mar 13 09:11:35 EST 1997
Can anyone tell me how 35S metabollic labelling time is determined? I have
read several variations from 15 minutes to several hours. I myself have
tried 15 minutes, 1 hour and 20 hours.
Also, when pre-incubating with methionine-free medium and subsequent
incubation with 35S-Met medium should fetal calf serum be included or not
i.e. does FCS contain methionine or cysteine?
Thanks in advance,
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