Metabolic labelling

L.Kempster l.kempster at
Thu Mar 13 09:11:35 EST 1997

Can anyone tell me how 35S metabollic labelling time is determined?  I have
read several variations from 15 minutes to several hours.  I myself have
tried 15 minutes, 1 hour and 20 hours.

Also, when pre-incubating with methionine-free medium and subsequent
incubation with 35S-Met medium should fetal calf serum be included or not
i.e. does FCS contain methionine or cysteine?

Thanks in advance,

Lee Kempster
Imperial College

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