western blot

M Allen M3ALLEN at BIOLOGY.Watstar.UWaterloo.CA
Thu Mar 13 17:05:16 EST 1997


Keld Sorensen wrote:
> 
> Depends on the detection system. If you use HRP both are equal, if you
> use AP, you should use TBS since phosphate is an inhibitor of
> PHOSPHATASE.

Hello netters
I'm relatively new to Western blotting, but our lab routinely uses PBS
and an AP detection.
The PBS is soaked out first however.
Would it make a significant difference to switch to a TBS system?
Thanks in advance
Mike Allen



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