western blot

M Allen M3ALLEN at BIOLOGY.Watstar.UWaterloo.CA
Thu Mar 13 17:05:16 EST 1997

Keld Sorensen wrote:
> Depends on the detection system. If you use HRP both are equal, if you
> use AP, you should use TBS since phosphate is an inhibitor of

Hello netters
I'm relatively new to Western blotting, but our lab routinely uses PBS
and an AP detection.
The PBS is soaked out first however.
Would it make a significant difference to switch to a TBS system?
Thanks in advance
Mike Allen

More information about the Methods mailing list