what is what I need

nhwu at PUBLIC3.BTA.NET.CN nhwu at PUBLIC3.BTA.NET.CN
Thu Mar 13 21:51:37 EST 1997


Hello everybody:

I am trying to find a RNA binding protein through gel retardation. The RNA 
fragment is synthesized in vitro. I first cut the plasmid which has the DNA 
template to make sure that the synthesis will stop automatically and then using 
T3 RNA polymerase synthesize the RNA fragment.So the RNA I synthesized ought to 
have the same length or be shorter. But in fact, after running 5% native gel, I 
found two bands. The one running ahead is stronger than the one running behind 
it.

So anyone who knows what happened please give me a reply. Thank you!

Tem





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