gel shift assay

[Toumy Guettouche]

On Wed, 12 Mar 1997, Joachim Kremerskothen wrote:

> Hi netters,
> 
> we have some problems with our gel shift assays because our protein/RNA
> complex do not run on a native 5% polyacrylamide gel using TBE buffer (pH
> 7,6). Any suggestions for another gels system, etc.?
> 
> Thanx in advance,
> 
>                               Joey
> 
> 
We usually use TGE buffer. It works better than TBE anyway. I actually 
don't know why people would use TBE buffer for gel shifts. Another buffer 
you can use is Tris-Taurine. You can find the receipe in the manual for 
the Sequenase Sequencing kit from Amersham. works like a charm.

Hope that helps.

toumy